Blood and Cell DNA Extraction (Automated)
Part Number: BC0016
$62.72
The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.
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The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.
The magnetiQ Blood & Cell DNA Extraction Kit also provides cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high-yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.
Applications
- Next Generation Sequencing (NGS)
- PCR and qPCR gene amplification
- DNA methylation studies
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates organic solvent hazardous waste
- Requires only the use of a magnetic rack and standard pipettes
The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
| Sample Type | Blood, Cells, Cell culture |
| Formats | Manual: 100 or 250 assays Automated: 16 or 96 assays |
| Elution Volume | 100 µL |
| DNA recovered | >98% |
| Processing Mode | Automated; Manual. Compatible with 96-well plates. |
| Throughput | 1-250 samples per run |
| Binding Technology | Magnetic beads |
| Binding Capacity | Scalable |
| Components | (i) Lysis/Binding Buffer (ii) Wash Buffer #1 (iii) Wash Buffer #2 (iv) Elution Buffer (v) Proteinase K |
| Storage | Room temperature |
| Kit Size | Product Code |
|---|---|
| 100 preps | BC0100 |
| 250 preps | BC0250 |
| 16 preps ready-to-use prefilled plates | BC0016 |
[Automated 16 Samples] Quick Start Guide: Blood & Cell Extraction Kit (BC0016)
Technical Specifications – Blood & Cell Extraction Kit
Video Protocol
DNA Extraction from Blood
DNA Extraction from Cells
The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.