Plant RNA Extraction (Manual)
Part Number: PR0050The magnetiQ Plant RNA Extraction Kit enables a rapid and straightforward nucleic acid isolation method from various plant samples, including tomato, tobacco, strawberry, cannabis, pine, mint, and lemon leaves. RNA extraction and purification is rapidly completed and easily amenable to high-throughput semi-automated workflow.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing.
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The magnetiQ Plant RNA Extraction Kit performs better than leading commercially available column-based kits. A high quantity of pure RNA is extracted from various plants using the magnetiQ Plant RNA Extraction Kit, resulting in lower CT values and efficient inhibitor and organic solvent removal.
The extraction kit has been validated with tomato, tobacco, strawberry, cannabis, pine, mint, and lemon leaves.
Applications
- Quantification via qPCR
- Next Generation Sequencing (NGS)
- Plant-Gender Screening
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Eliminates organic solvent hazardous waste
Contains optimized buffers for RNA binding, washing, and elution developed with Galenvs’ functionalized magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type | Plant samples |
Formats | Manual: 50 preps Prefilled plates: 16 preps |
Elution Volume | 100 µL |
DNA / RNA Recovered | >99% |
Processing Mode | Manual/Automated |
Throughput | 1-50 samples per run |
Binding Technology | Magnetic beads |
Binding Capacity | Scalable |
Components | (i) Lysis Buffer (ii) Binding buffer (iii) Wash Buffer #1 (iv) Wash Buffer #2 (v) Elution Buffer (vi) PPB (vii) DNase (viii) DNase Buffer (ix) Binding Buffer #2 (x) Bead Buffer (xi) Wash buffer #3 (xii) Bead Beating |
Storage | Room temperature |
Kit Size | Product Code |
---|---|
50 preps | PR0050 |
Prefilled plates 16-well | PR0016 |