Total RNA (Cell) Extraction (Automated)
Part Number: TRC0016The magnetiQ Total RNA (Cell) Extraction Kit enables a rapid and efficient method of extraction and purification of RNA and mRNA. DNA-free purification of RNA is achieved through magnetic bead-based capture and subsequent elution into a clean buffer. The kit alleviates the need for centrifugation steps and separate collection tubes – in contrast to standard column-based kits.
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The magnetiQ Total RNA Extraction Kit offers high yield and purity of RNA and mRNA. The purified sample can be concentrated in volumes as low as 50 μL, compared to column-based kits which often necessitate larger elution volumes. The magnetiQ Total RNA Extraction Kit performance demonstrates high purification efficacy at various fragment sizes. Sample processing is completed in less than 30 minutes, yielding high purity and efficient recovery of RNA and mRNA.
Applications
- Next Generation Sequencing (NGS)
- Real-time RT-PCR
- Microarray
- Transcriptome profiling and analysis
- Poly-A purification
- Northern Blot
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates organic solvent hazardous waste
Contains optimized buffers for RNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type | Cells, cell culture |
Formats | Manual: 100, 250 preps Prefilled plates: 16 preps |
Elution Volume | 50 µL or above |
Processing Mode | Automated; Manual. Compatible with 96-well plates. |
Throughput | 1-250 samples per run |
Binding Technology | Magnetic beads |
Binding Capacity | Scalable |
Components | (i) Binding Buffer (ii) Binding Magnetic Particles (iii) Wash Buffer 1 (iv) Wash Buffer 2 (v)Elution Buffer |
Storage | Room temperature |
Kit Size | Product Code |
---|---|
100 preps | TRC0100 |
250 preps | TRC0250 |
16 preps ready-to-use prefilled plates | TRC0016 |