Universal Pathogen DNA / RNA Extraction (Manual)
Part Number: UP0050
$196.00
The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.
One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:
- Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
- Serum/plasma
- Blood and Exudate
- Saliva samples
- Cell-free body fluids
- Complex matrices such as sputum or stool
- Cerebrospinal fluid (CSF)
The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).
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The magnetiQ Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process. The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.
The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. It does not require any additives such as proteinase K or RNA carrier.
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Applications
- Extraction of COVID-19 in clinical laboratories for the isolation and recovery of SARS-CoV-2 RNA
- Research and detection of viral and bacterial infection
- Quantification via Reverse Transcriptase qPCR (RT-qPCR)
- Next Generation Sequencing (NGS)
- Genomic DNA library preparation
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates need for Proteinase K treatment of swab samples
- Eliminates need for Carrier RNA in all sample types
- Eliminates centrifugation steps associated with spin-column based viral extraction kits
The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.
| Sample Type | Swab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF |
| Formats | Manual: 50, 200 preps Automated: 16 preps |
| Elution Volume | 50-100 µL |
| DNA / RNA Recovered | >99% |
| Processing Mode | Automated; Manual. Compatible with 96-well plates. |
| Throughput | 1-250 samples per run |
| Binding Technology | Magnetic beads |
| Binding Capacity | Scalable |
| Components | (i) Pathogen Lysis/Binding Buffer (ii) Binding Magnetic Nanoparticles (iii) Wash Buffer #1 (iv) Wash Buffer #2 (v) Elution Buffer |
| Storage | Room temperature |
| Kit Size | Product Code |
|---|---|
| 50 preps | UP0050 |
| 200 preps | UP0200 |
| 16 preps ready-to-use prefilled plates | UP0016 |
The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.
One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:
- Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
- Serum/plasma
- Blood and Exudate
- Saliva samples
- Cell-free body fluids
- Complex matrices such as sputum or stool
- Cerebrospinal fluid (CSF)
The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).