Wastewater DNA/RNA Extraction (Automated)

Part Number: WW0016
Wastewater DNA/RNA Extraction (Automated)
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$94.08

The magnetiQ Wastewater Extraction Kit provides an efficient and high-throughput RNA/DNA from influent wastewater with light to heavy sludge content. Up to 10 mL can be processed with simple manual or semi-automated protocols in under 40 minutes.

The kit utilizes functionalized and differentiated magnetic functional particles for the concentration of the pathogen from a large sample volume and isolation of nucleic acid eluted. Pure RNA and DNA are concentrated into 100 µL of elution buffer and can be used for microbial and pathogen detection, via qPCR, qRT-PCR and NGS depending on experimentation analysis needs.

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Description:

The magnetiQ Wastewater DNA/RNA Extraction Kit allows you to extract a high quantity of DNA/RNA from various microorganisms found in wastewater, including bacteria, fungi, algae, etc., resulting in a lower CT value.

RNA and DNA that have been extracted from the sample are concentrated in 100 µL of elution buffer and can be used for microbial and pathogen detection via qPCR, qRT-PCR, and next-generation sequencing (NGS).

Wastewater DNA/RNA Extraction

Applications

  • Next Generation Sequencing (NGS)
  • Quantification via qPCR, qRT-PCR

Features

  • Amenable to high-throughput methods, including automation
  • No Proteinase K Treatment
  • No Bead Beating Required

Contains optimized buffers for DNA/RNA binding, washing, and elution developed with Galenvs’ functionalized magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.

Sample Type Wastewater samples
Formats Manual: 100 preps
Prefilled plates: 16 preps, 24 preps, 96 preps
Elution Volume 100 µL
DNA / RNA Recovered >99%
Processing Mode Manual/Semi-Automated/Automated
Throughput 1-100 samples per run
Binding Technology Magnetic beads
Binding Capacity Scalable
Components (i) Concentration Buffer
(ii) Concentration Bead
(iii) Lysis Buffer
(iv) Binding Buffer
(v) Beads Buffer
(vi) Wash Buffer #1
(vii) Wash Buffer #2
(viii) Elution Buffer
Storage Room temperature
Kit Size Product Code
100 preps WW0100
16 preps ready-to-use prefilled plates WW0016
24 preps ready-to-use prefilled plates WW0024
96 preps ready-to-use prefilled plates WW0096