Wastewater DNA/RNA Extraction (Manual)
Part Number: WW0100
$490.00
The magnetiQ Wastewater Extraction Kit provides an efficient and high-throughput RNA/DNA from influent wastewater with light to heavy sludge content. Up to 10 mL can be processed with simple manual or semi-automated protocols in under 40 minutes.
The kit utilizes functionalized and differentiated magnetic functional particles for the concentration of the pathogen from a large sample volume and isolation of nucleic acid eluted. Pure RNA and DNA are concentrated into 100 µL of elution buffer and can be used for microbial and pathogen detection, via qPCR, qRT-PCR and NGS depending on experimentation analysis needs.
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The magnetiQ Wastewater DNA/RNA Extraction Kit allows you to extract a high quantity of DNA/RNA from various microorganisms found in wastewater, including bacteria, fungi, algae, etc., resulting in a lower CT value.
RNA and DNA that have been extracted from the sample are concentrated in 100 µL of elution buffer and can be used for microbial and pathogen detection via qPCR, qRT-PCR, and next-generation sequencing (NGS).
Applications
- Next Generation Sequencing (NGS)
- Quantification via qPCR, qRT-PCR
Features
- Amenable to high-throughput methods, including automation
- No Proteinase K Treatment
- No Bead Beating Required
Contains optimized buffers for DNA/RNA binding, washing, and elution developed with Galenvs’ functionalized magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
| Sample Type | Wastewater samples |
| Formats | Manual: 100 preps Prefilled plates: 16 preps, 24 preps, 96 preps |
| Elution Volume | 100 µL |
| DNA / RNA Recovered | >99% |
| Processing Mode | Manual/Semi-Automated/Automated |
| Throughput | 1-100 samples per run |
| Binding Technology | Magnetic beads |
| Binding Capacity | Scalable |
| Components | (i) Concentration Buffer (ii) Concentration Bead (iii) Lysis Buffer (iv) Binding Buffer (v) Beads Buffer (vi) Wash Buffer #1 (vii) Wash Buffer #2 (viii) Elution Buffer |
| Storage | Room temperature |
| Kit Size | Product Code |
|---|---|
| 100 preps | WW0100 |
| 16 preps ready-to-use prefilled plates | WW0016 |
| 24 preps ready-to-use prefilled plates | WW0024 |
| 96 preps ready-to-use prefilled plates | WW0096 |
The magnetiQ Wastewater Extraction Kit provides an efficient and high-throughput RNA/DNA from influent wastewater with light to heavy sludge content. Up to 10 mL can be processed with simple manual or semi-automated protocols in under 40 minutes.
The kit utilizes functionalized and differentiated magnetic functional particles for the concentration of the pathogen from a large sample volume and isolation of nucleic acid eluted. Pure RNA and DNA are concentrated into 100 µL of elution buffer and can be used for microbial and pathogen detection, via qPCR, qRT-PCR and NGS depending on experimentation analysis needs.